This project aims to develop a biomimetic skin-like platform based on microfluidic technology, which can replace the human-involved experiment of extracting interstitial fluid through electroosmosis. By introducing multi-layer micro-structured chips and precise electric field control systems, it enables the controllable simulation of interstitial fluid transmembrane migration, providing an ethics-free and highly reliable in vitro testing platform.
How it is being developed
The present invention has constructed a skin-like chip simulation platform based on microfluidic technology. It forms a closed system by combining a peristaltic pump with multi-layer biomimetic structure chip modules, effectively simulating the extraction process of interstitial fluid in the human body. The chip, from top to bottom, includes an imitation skin layer, a simulated cortex layer, a cell cytoplasmic matrix simulation layer, and an interstitial fluid circulation layer, respectively reproducing key characteristics such as skin barrier, liquid enrichment, physiological resistance, and dynamic transport. The platform uses a peristaltic pump with adjustable speed (flow rate 0.2 μL/min) to precisely control the flow rate, which is highly matched with human physiological parameters, achieving a true reproduction of interstitial fluid transmembrane migration, providing a high-simulation and ethically risk-free in vitro verification scheme for transdermal extraction technology.
Expected outcome
A highly realistic in vitro simulation platform for skin-like tissues has been constructed, which can effectively replace animal or human experiments and be applied to research related to interstitial fluid extraction. This platform precisely simulates the physiological structure and permeation characteristics of human skin through multi-layer microfluidic structures and biomimetic membrane materials and truly reproduces the transmembrane migration process of interstitial fluid under the action of electric fields. The platform has non-invasive verification capabilities and provides an experimental basis for optimizing the electrode structure and material parameters of electroosmosis extraction. At the same time, this platform can be widely used in the testing and verification of wearable health monitoring devices and biochips, promoting the clinical transformation and industrialization of counter-ion electroosmosis technology.
Brief Introduction
This project aims to develop a biomimetic skin-like platform based on microfluidic technology, which can replace the human-involved experiment of extracting interstitial fluid through electroosmosis. By introducing multi-layer micro-structured chips and precise electric field control systems, it enables the controllable simulation of interstitial fluid transmembrane migration, providing an ethics-free and highly reliable in vitro testing platform.
How it is being developed
The present invention has constructed a skin-like chip simulation platform based on microfluidic technology. It forms a closed system by combining a peristaltic pump with multi-layer biomimetic structure chip modules, effectively simulating the extraction process of interstitial fluid in the human body. The chip, from top to bottom, includes an imitation skin layer, a simulated cortex layer, a cell cytoplasmic matrix simulation layer, and an interstitial fluid circulation layer, respectively reproducing key characteristics such as skin barrier, liquid enrichment, physiological resistance, and dynamic transport. The platform uses a peristaltic pump with adjustable speed (flow rate 0.2 μL/min) to precisely control the flow rate, which is highly matched with human physiological parameters, achieving a true reproduction of interstitial fluid transmembrane migration, providing a high-simulation and ethically risk-free in vitro verification scheme for transdermal extraction technology.
Expected outcome
A highly realistic in vitro simulation platform for skin-like tissues has been constructed, which can effectively replace animal or human experiments and be applied to research related to interstitial fluid extraction. This platform precisely simulates the physiological structure and permeation characteristics of human skin through multi-layer microfluidic structures and biomimetic membrane materials and truly reproduces the transmembrane migration process of interstitial fluid under the action of electric fields. The platform has non-invasive verification capabilities and provides an experimental basis for optimizing the electrode structure and material parameters of electroosmosis extraction. At the same time, this platform can be widely used in the testing and verification of wearable health monitoring devices and biochips, promoting the clinical transformation and industrialization of counter-ion electroosmosis technology.